Just got a dozen arrows in and shot one but noticed that the the knock was on wrong to where the songle colored fletch was not striaght out(-) 9 o'clock, does it matter or does it need to be corrected?

Whether the cock vane is up, down, or sideways is largely a function of the rest you are using. If you need that vane in a different direction, it won't hurt to turn the nock to get the orientation you need.

So with a whisker biscuit it should not matter.

Depends on the biscuit. If it's the older style (all black) it won't matter, if it's the new style (brown with black bottom) then the cock fletching should be up as to not have any fletchings pass thru the black part of the biscuit.

I shoot all ofmy arrows with the cock vain up through my whisker biscuit, if for nothing else consistency.