I had a nice buck at 18 yards (http://forum.hunting.net/asppg/tm.asp?m=810431) and he spooked. My question is: should I do some calling right away as the deer is trotting/running away or be quiet?

I hit the can call almost immediately and then followed it with a young buck grunt. Didn't work. Will calling the buck turn him off to other calling (will he assoiate the calls with what spooked him)?????