Heck, I see these silly signs along the road all the time "Deer Crossing". I figure if they are smart enough to read the signs, they will catch on!
Seriously, No matter how you define the borders, if the critters dont like the land, they dont use it. If they do, they do. Expect it to take potentially years for them to adapt to the change. They undoubtedly have places they already are used to and use. I would be sure to avoid the new planted areas as much as possible while the zone gets established.