If the urine was any fresher, the doe herself would have been there peeing on the ground herself!!!!!!! I got it with an hour of being collected and immediately put in the frig!!!! I get it home and keep it in the frig. I take it out and put it in a cooler to the hunting site and take it out and put it on the drag rag and in we go. I put the bottle back in cooler at the truck. Just whats in the glass jar is all I use for the hunt. I get 2 hunts out of a 2oz bottle. Wash out drag rag after 2nd hunt and trade out drag rags for others left out to air.