when we start hunting in the fall ,the gang i hunt with. when the first deer dies we start collecting deer urine,buck ,doe it doesn't matter. we put it all in a big jar in the frig. as more deer die ,we keep adding to the jar. when the rut gets close and scent glands start to darken up we will cut the dark hair from them and drop it in the urine. then just keep adding and drain off what you want to use. with us we may end up with a gallon or so in a season. last year i had made scapes useing this urine in them, almost every deer that would come by would stop and freshen the scrapes