I am fairly new to using doe estrous. I used it twice last year and have used it once this year, and it seems to work during late pre-rut and the rut.

I am using Code Blue estrous doe pee, which is taken from a single deer, which makes sense to me. I have three questions:

1) I used probably at least a third of a bottle around my stand (in it all day)a week ago and won't have a chance to hunt until at least next weekend. I have kept the pee in the original bottle with the spray nozzle on it. Do you think it will have "gone bad" or "old" by next week under those circumstances? It's been kept mostly at room temperature.

2) It seems to me most of the doe pursuits I have seen in the field take place in the afternoon, not the morning. Have any of you noticed anything time-of-day sensitive about when bucks are inclined to chase does?

3) Should I "renew" the wicks or spray trails around my stand if I'm in the stand all day? If so, how often?

Thanks for any help!