I have a amerstep blind that has a floor. the floor can be unzipped to get it out of the way. While carpet and other things have been suggest if it isn't sealed tight to the blind, spiders can still get in and then you will have a fear crazed woman clawing and other stuff to get out.

Ortho home defense spray last for a while, get it by the gallon. Use a leaf rake to clean the area up of any litter then spray inside and around the outside edges of a floorless blind.
That should do the trick and tell wifey to watch for deer and not for spiders on the ground.

Al