Yes, we have sprayed roundup on kura clover at work since 2006 with no appreciable loss. There is also alfalfa and red clover in the fields, and they survive as well. It slows the clover for about 2 weeks, and you will ever know it was sprayed 1 month later. It also will not kill tough broadleaf weeds like dandelions, but seeding weeds and grasses will go down fast. I would only try it on a well established (2 yrs old +) perennial clover field in spring that is not under stress.