I prefer standing, but you just never know. Last year I had nice 10 point come from behind me, he stood under the tree then moved forward and was starting to veer off to the left (bad direction in this case) I mouth grunted, stopped him and took him from the sitting position. I felt it much more important to not spook the animal and remain seated and think through the shot. It is a slightly different feel (anchor point) when sitting, but a slight adjustment and it' s pretty much the same...to me at least. I will typically take a few practice shots sitting. I' m glad I did last year! :-)