Sure, without a doubt. Since the area was walked all around for 4 hours, that's plenty of disturbance and leaving scent behind, but that activity was spread around a big area. If the disturbance was focused in a small spot, like a 1/4 acre, then yes, the does may not like it and skirt around that spot. Since the disturbance was wide and only one time, no one spot should concern the deer there. This also depends on how smart the lead doe is too, but most your mature bucks wouldn't tolerate it and wouldn't make it a place to visit unless it's under darkness.

iSnipe