As stated previously, 4" below the spine (directly above the front legs) will drop them where the stand. I've took my first buck with this shot using a 12 ga rifled slug and my second buck with a .30-06. The 180gr .30-06 round ruined the some of the backstraps so I stopped taking that shot with a rifle. Last year I shot my first buck with a handgun and used that shot with a 44mag 240 gr bullet. It fell where it stood.