Standing as often as possible but I have made some shots sitting.I have shot many more practice arrows standingthansitting over the last 26 years of shooting bows and in actual hunting situations. Standing offers me better positioning options as the deer are moving through woods etc and you just never know when you will be able to take that best shot. Sitting limits you in being able to shoot at a broader radius and from diffreent agles etc.

Not saying either is right or wrong, just saying how I like to shoot from my tree stands.