I have used real urine straight from the bladder and tarsel glands. Your probably wondering how to attract the deer in order to be able to use these items.Here's what I do. I take a tampon and tie a loop in the string,then I get a suitable donor speciman and put them in 35mm film canisters. I surround my stand with the tampons hanging them on low branches. When I'm done for the day I put them back in the canisters and secure them in the freezer. I have shot many deer while at these cotton candies.