I've seen this happen 100 times. A doe who is not in heat will almost turn inside out trying to get away from a doe who is in heat. Why? Because, she does not want to be harrassed and chased by a big horny buck when he gets a snootfull of a hot doe.
I have found that live does make a better attractant for big bucks than ANY pee-pee in a bottle. If you really think you need to use some magic estrous scent, and if it will give you the confidence you need to stay out there longer, cut it at least 50% with regular doe urine. This does not seem to put the does on alert nearly as bad, and bucks will still come to investigate.