Yes, you can take it off after you get your anchor point down.

Here is a little tip:

The more anchor points you have, the more consistant you will be. Think of it like a graph. If you only have one point, the line can go any direction. If you have two points you will see the slope of the line, but you may not be able to follow the graph consistantly. If you have twenty dots, you will have a better chance of finding the slope.

Now what does this have to do with bowhunting you may ask. If you only rely on your kisser button, (not saying you do) you won't be as consistant as you would with more anchor points. I anchor by putting my nose on the string, touching the string to the corner of my mouth, and put my knuckle on my ear lobe. It is almost impossible to not have a consistant anchor point by using several anchor points.